In situ probing of microbial activity within anammox granular biomass with microelectrodes
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Beside biofilm, granules with average diameter of approximately 5 mm formed in an AnRBC.

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In situ microbial activity of anammox and denitrification within granules were investigated with microelectrodes.

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The most active zone of anammox was in the surface layer of 200–400 μm, and anammox activity increased with increasing of substrate concentration.

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Denitrification activity was identified and the most active zone was limited to the inner layer of the granules.

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