Standardization of the manufacturing procedure for Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine

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• 作者：Tao Su^a ; ^c ; ^{12467324@life.hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Wei-Wei Zhang^a ; ^{zhangww7hk@126.com" class="auth_mail" title="E-mail the corresponding author} ; Ya-Ming Zhang^a ; ^{yasmine@hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Brian Chi-Yan Cheng^a ; ^{brichian@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Xiu-Qiong Fu^a ; ^{13480405@life.hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Ting Li^a ; ^{14485451@life.hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Hui Guo^a ; ^{14485265@life.hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Ya-Xi Li^a ; ^{15485056@life.hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Pei-Li Zhu^a ; ^{15485528@life.hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Hui Cao^b ; ^{kovhuicao@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Zhi-Ling Yu^a ; ^c ; ^{zlyu@hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ATCC ; American Type Culture Collection ; CMM ; Chinese medical material ; CP ; Chinese pharmacopoeia ; DMSO ; Dimethyl sulphoxide ; DMEM ; Dulbecco&rsquo ; s Modified Eagle Medium ; ESI ; Electrospray ionization ; FBS ; Fetal bovine serum ; FA ; Formic acid ; HPLC ; High performance liquid chromatography ; PR ; Pinelliae Rhizoma ; PRZA ; Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine ; TCM ; traditional Chinese medicine ; UPLC/Q-TOF-MS ; Ultra-performance liquid chromatography/quadrupole-time- of-flight mass spec
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：663-669
• 全文大小：708 K

文摘

Pinelliae Rhizoma (PR), the dried tuber of Pinellia ternata (Thunb.) Breit., is a traditional Chinese medicinal herb. It is commonly used for treating cancer, cough and phlegm. To treat cancer, Chinese medicine practitioners often use raw PR; while to treat cough and phlegm, they usually use Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine (PRZA, raw PR processed with ginger juice and alumen as adjuvant materials). Currently, the producing protocol of PRZA varies greatly among different places in China. This study aims to standardize the manufacturing procedure for PRZA. We also evaluated the impact of processing on the bioactivities and chemical profile of raw PR.

Materials and methods

We used the orthogonal design to optimize the manufacturing procedure of PRZA at bench scale, and validated the optimized procedure in pilot-scale production. The MTT assay was used to compare the cytotoxicities of raw PR and PRZA in hepatocellular carcinoma HepG2 cells. Animal models (ammonia liquor-induced cough model and phenol red secretion model) were used to compare the antitussive and expectorant effects of raw PR and PRZA, respectively. The chemical profiles of raw PR and PRZA samples were compared using a newly developed ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method.

Results

The standardized manufacturing procedure for PRZA is as follows: soak raw PR in water until the center of the cut surface is devoid of a dry core, after that, boil the herb in water (for each 100 kg raw PR, 12.5 kg alumen and 25 L freshly squeezed ginger juice are added) for 6 h, and then take out and dry them. The cytotoxicity of PRZA was less potent than that of raw PR. Intragastric administration of raw PR or PRZA demonstrated antitussive and expectorant effects in mice. These effects of PRZA were more potent than that of raw PR at the dose of 3 g/kg. By comparing the chemical profiles, we found that six peaks were lower, while nine other peaks were higher in PRZA than in raw PR. Six compounds corresponding to six individual changed peaks were tentatively identified by matching with empirical molecular formulae and mass fragments.

Conclusion

The manufacturing procedure for PRZA was standardized. This protocol can be used for PRZA industrial production. The bioactivity assay results of raw PR and PRZA (produced using the standardized protocol) support the common practice for the clinical applications of these two decoction pieces. Moreover, raw PR and PRZA showed different chemical profiles. Further studies are warranted to establish the relationship between the alteration of chemical profiles and the changes of medicinal properties caused by processing.