A smartphone based surface plasmon resonance imaging (SPRi) platform for on-site biodetection

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• 作者：Hasan Guner^aAuthor Vitae ; Erol Ozgur^aAuthor Vitae ; Guzin Kokturk^bAuthor Vitae ; Mehmet Celik^cAuthor Vitae ; Elif Esen^bAuthor Vitae ; Ahmet E. Topal^aAuthor Vitae ; Sencer Ayas^dAuthor Vitae ; Yildiz Uludag^bAuthor Vitae ; Caglar Elbuken^aAuthor Vitae ; Aykutlu Dana^a ; ^{aykutlu@unam.bilkent.edu.tr" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Plasmonics ; Surface plasmon resonance imaging ; Optical discs ; Mobile sensing ; Biosensors ; Optical sensor
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：239
• 期：Complete
• 页码：571-577
• 全文大小：1473 K

文摘

We demonstrate a surface plasmon resonance imaging platform integrated with a smartphone to be used in the field with high-throughput biodetection. Inexpensive and disposable SPR substrates are produced by metal coating of commercial Blu-ray discs. A compact imaging apparatus is fabricated using a 3D printer which allows taking SPR measurements from more than 20.000 individual pixels. Real-time bulk refractive index change measurements yield noise equivalent refractive index changes as low as 4.12 × 10⁻⁵ RIU which is comparable with the detection performance of commercial instruments. As a demonstration of a biological assay, we have shown capture of mouse IgG antibodies by immobilized layer of rabbit anti-mouse (RAM) IgG antibody with nanomolar level limit of detection. Our approach in miniaturization of SPR biosensing in a cost-effective manner could enable realization of portable SPR measurement systems and kits for point-of-care applications.