RNAi Reveals Phase-Specific Global Regulators of Human Somatic Cell Reprogramming
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- 作者：Cheng-Xu Delon Toh1 ; 2 ; 24 ; Jun-Wei Chan1 ; 24 ; Zheng-Shan Chong1 ; 24 ; Hao Fei Wang1 ; 3 ; Hong Chao Guo1 ; 4 ; Sandeep Satapathy1 ; Dongrui Ma5 ; Germaine Yen Lin Goh6 ; Ekta Khattar7 ; Lin Yang8 ; 9 ; 10 ; Vinay Tergaonkar7 ; 11 ; Young-Tae Chang12 ; 13 ; James J. Collins10 ; 14 ; 15 ; 16 ; George Q. Daley10 ; 17 ; 18 ; 19 ; 20 ; Keng Boon Wee21 ; 22 ; Chadi A. EL Farran1 ; 3 ; Hu Li23 ; li.hu@mayo.edu" class="auth_mail" title="E-mail the corresponding author ; Yoon-Pin Lim2 ; 11 ; 22 ; bchlyp@nus.edu.sg" class="auth_mail" title="E-mail the corresponding author ; Frederic A. Bard6 ; 11 ; Yuin-Han Loh1 ; 2 ; 3 ; yhloh@imcb.a-star.edu.sg" class="auth_mail" title="E-mail the corresponding author
- 关键词：human somatic cell reprogramming ; genome-wide siRNA screen ; SFRS11 ; reprogramming specific alternative splicing ; ZNF207
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：21 June 2016
- 年：2016
- 卷：15
- 期：12
- 页码：2597-2607
- 全文大小：4410 K
文摘
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Key functional regulators of human somatic cell reprogramming are identified
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Combinatorial knockdown of five repressors allows highly efficient reprogramming (85%)
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SFRS11 regulates splicing of genes that are critical for reprogramming
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Splicing of downstream ZNF207 isoform B to A and C affects reprogramming efficiency