Deulajhari hot spring is located in
the Angul district of Odisha. The significance of this hot spring is
the presence of
the hot spring cluster adjacent to
the cold spring which attracts
the attention of microbiologists to understand
the role of physio-chemical factors of
these springs on bacterial community structure. Next-generation sequencing technology helps us to depict
the pioneering microflora of any ecological niche based on metagenomic approach. Our study represents
the first Illumina based metagenomic study of Deulajhari hot spring DH1, and DH2 of
the cluster with temperature 65 °C to 55 °C respectively establishing a difference of 10 °C. Comprehensive study of microbiota of
these two hot springs was done using
the metagenomic sequencing of 16S rRNA of V3‐V4 region extracting metagenomic DNA from
the two hot spring sediments. Sequencing community DNA reported about 28 phyla in spring DH1 of which
the majority were Chloroflexi (22.98%), Proteobacteria (15.51%), Acidobacteria (14.51%), Chlorobi (9.52%), Nitrospirae (8.54%), and Armatimonadetes (7.07%), at
the existing physiochemical conditions like; temperature 65 °C, pH 8.06, electro conductivity 0.020 dSm
− 1, and total organic carbon (TOC) 3.76%. About 40 phyla were detected in cluster DH2 at
the existing physiochemical parameters like temperature 55 °C, pH 8.10, electro conductivity 0.019 dSm
− 1, and total organic carbon (TOC) 0.58% predominated with Chloroflexi (41.98%), Proteobacteria (10.74%), Nitrospirae (10.01%), Chlorobi (8.73%), Acidobacteria (6.73%) and Planctomycetes (3.73%). Approximately 68
class, 107 order, 171 genus and 184 species were reported in cluster DH1 but 102
class, 180 order, 375 genus and 411 species in cluster DH2. The comparative metagenomics study of
the Deulajhari hot spring clusters DH1, and DH2 depicts
the differential profile of
the microbiota.
Metagenome sequences of these two hot spring clusters are deposited to the SRA database and are available in NCBI with accession no. SRX1459734 for DH1 and SRX1459735 for DH2.