Identification, biochemical characterization, and in-vivo expression of the intracellular invertase BfrA from the pathogenic parasite Leishmania major
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- 作者：Sorya Belaza ; b ; 1 ; Thibault Rattierc ; 1 ; Pierre Lafitec ; Philippe Moreauc ; Franç ; oise H. Routierd ; Florence Robert-Gangneuxa ; b ; Jean-Pierre Gangneuxa ; b ; Richard Daniellouc ; richard.daniellou@univ-orleans.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Invertase ; Sucrose ; Leishmania ; Glycosylation ; Leishmaniasis
- 刊名：Carbohydrate Research
- 出版年：2015
- 出版时间：13 October 2015
- 年：2015
- 卷：415
- 期：Complete
- 页码：31-38
- 全文大小：858 K
文摘
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A sucrose-metabolizing invertase from the protozoan pathogen Leishmania major was identified.
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RT-PCR experiments demonstrated that this enzyme was expressed in other Leishmania species.
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The expression is dependent on the parasite life stage (overexpressed in promastigotes).
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The cloned and expressed recombinant enzyme has a low affinity for sucrose yet comparable to other invertases.
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These results, coupled to molecular modeling and sequence analysis, suggest that this invertase is an intracellular enzyme.