Comparison of three extraction methods to detect noroviruses in dairy products
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- 作者：Catherine Hennechart-Collette ; Sandra Martin-Latil ; Audrey Fraisse ; Sylvie Perelle ; sylvie.perelle@anses.fr" class="auth_mail" title="E-mail the corresponding author
- 刊名：Food Microbiology
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：61
- 期：Complete
- 页码：113-119
- 全文大小：559 K
文摘
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We provide a rapid and sensitive method for detecting NoV in milk and cottage cheese.
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The recovery efficiencies were of 54.87%–98.87% for NoV GI and 61.16%–96.50% for NoV GII.
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We use the Murine Norovirus (MNV-1) and Mengovirus as a process control.
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MNV-1 and mengovirus offers a reliable way to monitor the quality of the extraction procedures.
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The limit of detection was in the range of 103 and 105 genome copies per sample respectively for NoVGII and NoVGI.