Biomimetic whitlockite inorganic nanoparticles-mediated in situ remodeling and rapid bone regeneration

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• 作者：Hwan D. Kim^a ; ¹ ; Hae Lin Jang^b ; ¹ ; Hyo-Yong Ahn^b ; Hye Kyoung Lee^b ; Jungha Park^a ; Eun-seo Lee^a ; Eunjee A. Lee^a ; Yong-Hoon Jeong^c ; Do-Gyoon Kim^c ; Ki Tae Nam^b ; ^{nkitae@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Nathaniel S. Hwang^a ; ^{nshwang@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Whitlockite ; Nanoparticles ; Inorganic phosphate ; Cryogel ; Bone tissue engineering ; Ion metabolic pathway ; Bone remodeling process
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：31-43
• 全文大小：3517 K

文摘

Bone remodeling process relies on complex signaling pathway between osteoblasts and osteoclasts and control mechanisms to achieve homeostasis of their growth and differentiation. Despite previous achievements in understanding complicated signaling pathways between cells and bone extracellular matrices during bone remodeling process, a role of local ionic concentration remains to be elucidated. Here, we demonstrate that synthetic whitlockite (WH: Ca₁₈Mg₂(HPO₄)₂(PO₄)₁₂) nanoparticles can recapitulate early-stage of bone regeneration through stimulating osteogenic differentiation, prohibiting osteoclastic activity, and transforming into mechanically enhanced hydroxyapatite (HAP)-neo bone tissues by continuous supply of PO₄³⁻ and Mg²⁺ under physiological conditions. In addition, based on their structural analysis, the dynamic phase transformation from WH into HAP contributed as a key factor for rapid bone regeneration with denser hierarchical neo-bone structure. Our findings suggest a groundbreaking concept of ‘living bone minerals’ that actively communicate with the surrounding system to induce self-healing, while previous notions about bone minerals have been limited to passive products of cellular mineralization.