IL-10 reduces apoptosis and extracellular matrix degradation after injurious compression of mature articular cartilage

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• 作者：P. Behrendt^&dagger ; ; ^{peter.behrendt@uksh.de" class="auth_mail" title="E-mail the corresponding author} ; A. Preusse-Prange^&Dagger ; ; ^{a.preusse@anat.uni-kiel.de" class="auth_mail" title="E-mail the corresponding author} ; T. Klü ; ter^&dagger ; ; ^{tim.klueter@uksh.de" class="auth_mail" title="E-mail the corresponding author} ; M. Haake^&Dagger ; ; ^{marina.haake@web.de" class="auth_mail" title="E-mail the corresponding author} ; B. Rolauffs^§ ; ; ^‖ ; ^{brolauffs@bgu-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; A.J. Grodzinsky^¶ ; ; ^{alg@mit.edu" class="auth_mail" title="E-mail the corresponding author} ; S. Lippross^&dagger ; ; ^{sebastian.lippross@uksh.de" class="auth_mail" title="E-mail the corresponding author} ; B. Kurz^&Dagger ; ; ^{bkurz@anat.uni-kiel.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cartilage ; IL-10 ; Injurious compression ; Osteoarthritis
• 刊名：Osteoarthritis and Cartilage
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：24
• 期：11
• 页码：1981-1988
• 全文大小：893 K

文摘

The aim of this study was to examine whether anti-inflammatory interleukin-10 (IL-10) exerts chondroprotective effects in an in vitro model of a single mechanical injury of mature articular cartilage.

Method

Articular cartilage was harvested from the femoro-patellar groove of adult cows (Bos taurus) and cultured w/o bovine IL-10. After 24 h of equilibration explants were subjected to an axial unconfined compression (50% strain, velocity 2 mm/s, held for 10 s). After 96 h cell death was measured histomorphometrically (nuclear blebbing, NB) and the release of glycosaminoglycans (GAG, DMMB assay) and nitric oxide (NO, Griess-reagent) were analyzed. mRNA levels of matrix degrading enzymes and nitric oxide synthetase were measured by quantitative real time PCR. Differences between groups were calculated using a one-way ANOVA with a Bonferroni post hoc test.

Results

Injurious compression significantly increased the number of cells with NB, release of GAG and nitric oxide and expression of MMP-3, -13, ADAMTS-4 and NOS2. Administration of IL-10 significantly reduced the injury related cell death and release of GAG and NO, respectively. Expression of MMP-3, -13, ADAMTS-4 and NOS2 were significantly reduced.

Conclusion

Joint injury is a complex process involving specific mechanical effects on cartilage as well as induction of an inflammatory environment. IL-10 prevented crucial mechanisms of chondrodegeneration induced by an injurious single compression. IL-10 might be a multipurpose drug candidate for the treatment of cartilage-related sports injuries or osteoarthritis (OA).