Cloning, heterologous expression, and enzymatic characterization of a novel glucoamylase GlucaM from Corallococcus sp. strain EGB
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- 作者：Zhoukun Lia ; Kai Jia ; Weiliang Donga ; Xianfeng Yea ; Jiale Wua ; Jie Zhoua ; Fei Wanga ; b ; Qiongzhen Chena ; Lei Fua ; Shuhuan Lia ; Yan Huanga ; Zhongli Cuia ; czl@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Corallococcus ; Cloning ; Glucoamylase ; Glucosidic bands
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：122-127
- 全文大小：851 K
文摘
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GlucaM shared no significant similarity with these glucoamylases (<10% identity), indicating that GlucaM may be a novel glucoamylase.
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The activity of GlucaM was independent of Ca2+.
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GlucaM is also highly resistant to high salt concentrations and various detergents.
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rGlucaM could act on different substrates and maltooligosaccharides, making it a good catalyst for production of glucose