Extraction, characterization and antioxidant activities of polysaccharides of Chuanminshen violaceum

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• 作者：Hongmin Dong^a ; ^{donghmin@outlook.com" class="auth_mail" title="E-mail the corresponding author} ; Qing Zhang^a ; ^{zhangqing@sicau.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yu Li^a ; ^{15283522791@163.com" class="auth_mail" title="E-mail the corresponding author} ; Lu Li^a ; ^{765416741@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Weijie Lan^a ; ^{lwj18728158690@163.com" class="auth_mail" title="E-mail the corresponding author} ; Jingliu He^a ; ^{hjingliu@163.com" class="auth_mail" title="E-mail the corresponding author} ; Huiyan Li^a ; ^{Lee909293@163.com" class="auth_mail" title="E-mail the corresponding author} ; Yabo Xiong^b ; ^{xiongyabo1030@163.com" class="auth_mail" title="E-mail the corresponding author} ; Wen Qin^a ; ^{qinwen@sicau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chuanminshen violaceum ; Polysaccharide ; Microwave-assisted extraction
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：86
• 期：Complete
• 页码：224-232
• 全文大小：2291 K

文摘

A single factor experiment and Box-Behnken design were applied to optimize the microwave-assisted extraction (MAE) of Chuanminshen violaceum polysaccharides (CVPs). The CVPs yield was 34.59 ± 0.51%, which was in accordance with the predicted value of 35.54%, under the following optimum conditions: microwave power of 466 W, extraction temperature of 64.5 °C, extraction time of 15 min, and water-raw material ratio of 40 mL/g. Two CVPs fractions (CVPs-I and CVPs-II) were obtained via stepwise ethanol precipitation. CVPs-II was further purified using DEAE cellulose-52 chromatography to obtain the major fraction of CVPs-IIa. Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis revealed that crude CVPs, CVPs-I, and CVPs-II were neutral polysaccharides and were mainly composed of glucose and galactose. The molecular weights of CVPs-I and CVPs-IIa were 233.69 and 11.02 kDa, respectively. Furthermore, all samples exhibited certain antioxidant activity or reducing power to scavenge the DPPH and ABTS radicals, among which CVPs-II was the strongest. In conclusion, MAE is an efficient method for extracting CVPs of C. violaceum, and this plant root has the potential to be explored as a source of natural antioxidants.