The EPSPS Pro106Ser substitution solely accounts for glyphosate resistance in a goosegrass (Eleusine indica) population from Tennessee, United States

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• 作者：L Huffman Janel^a ; ^{jlhuffm2@illinois.edu" class="auth_mail" title="E-mail the corresponding author} ; Chance W Riggins^a ; Lawrence E Steckel^b ; Patrick J Tranel^a ; ^{tranel@illinois.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：herbicide resistance ; monogenic trait ; glyphosate ; PCR amplification of specific alleles (PASA) ; EPSPS ; target-site mutation
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：15
• 期：6
• 页码：1304-1312
• 全文大小：731 K

文摘

Previous studies have documented the occurrence of glyphosate-resistant (GR) goosegrass (Eleusine indica (L.) Gaertn.) and, in at least some cases, resistance is due to an altered target site. Research was performed to determine if an altered target site was responsible for GR in a Tennessee, United States goosegrass population (TennGR). DNA sequencing revealed a mutation in TennGR plants conferring the Pro106Ser 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) substitution previously identified in other GR populations. F₂ populations were derived from TennGR plants crossed with plants from a glyphosate-susceptible population (TennGS) and analyzed for their response to glyphosate and genotyped at the EPSPS locus. Plants from the F₂ populations segregated 1:2:1 sensitive:intermediate:resistant in response to a selective dose of glyphosate, and these responses co-segregated with the EPSPS genotypes (PP106, PS106, and SS106). To separately investigate the effect of the Pro106Ser substitution on GR, glyphosate dose-response curves and 50% effective dose (ED₅₀) values were compared among the three genotypes and the two parental populations. The SS106 genotype was 3.4-fold resistant relative to the PP106 genotype, identical to the resistance level obtained when comparing the resistant and susceptible parental populations. We conclude that the mutation conferring a Pro106Ser EPSPS mutation is solely responsible for GR in the TennGR goosegrass population.