Chondroitin/chitosan nanoparticles with/without calcitonin were characterized.
Chondroitin sulfate can form binary complexes with calcitonin at the nanoscale.
Properties of chondroitin nanoplexes depend on the medium.
Media and composition of the nanoplexes affect the release of sCT.
Chondroitin nanoplexes are promising carriers for biomedical applications.