Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS
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- 作者：Marisa Kamelgarna ; b ; 1 ; meka229@g.uky.edu" class="auth_mail" title="E-mail the corresponding author ; Jing Chena ; 1 ; jchen4@email.uky.edu" class="auth_mail" title="E-mail the corresponding author ; Lisha Kuanga ; lisha.kuang@uky.edu" class="auth_mail" title="E-mail the corresponding author ; Alexandra Arenasa ; b ; maar223@g.uky.edu" class="auth_mail" title="E-mail the corresponding author ; Jianjun Zhaia ; jianjunzhai@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Haining Zhua ; b ; c ; haining@uky.edu" class="auth_mail" title="E-mail the corresponding author ; Jozsef Gala ; jgal2@uky.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ALS ; amyotrophic lateral sclerosis ; fALS ; familial ALS ; sALS ; sporadic ALS ; 293T ; human embryonic kidney (HEK293T) cells ; FUS/TLS ; Fused in Sarcoma/Translocated in Liposarcoma ; GST ; glutathione S-transferase ; RIPA ; radioimmunoprecipitation assay buffer ; SDS-PAGE ; denaturing polyacrylamide gel electrophoresis ; TBST ; TRIS-buffered saline/Tween-20 buffer ; PBS ; phosphate-buffered saline buffer ; EGFP ; enhanced green fluorescent protein ; BSA ; bovine serum albumin ; DAPI ; 4&prime ; 6-diamidino-2-phenylin
- 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：1862
- 期：10
- 页码：2004-2014
- 全文大小：1422 K
文摘
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We performed proteomic analysis of FUS interacting proteins from human cells.
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FUS interacts with numerous regulators of gene expression and RNA metabolism.
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The interactions were differentially impacted by RNase treatment.
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FUS interacted with Matrin-3 and hnRNPA1, proteins also involved in ALS pathogenesis.
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FUS interacted with numerous exosome components and was itself secreted by exosomes.