Sensitive biomolecule detection in lateral flow assay with a portable temperature-humidity control device
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- 作者：Jane Ru Choia ; b ; c ; Jie Hub ; c ; Shangsheng Fengb ; d ; e ; Wan Abu Bakar Wan Abasa ; Belinda Pingguan-Murphya ; bpingguan@um.edu.my" class="auth_mail" title="E-mail the corresponding author ; Feng Xub ; c ; fengxu@mail.xjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：LFA ; POC ; Nucleic acid hybridization ; Antigen&ndash ; antibody interaction ; Temperature ; RH ; Portable temperature&ndash ; humidity control device
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：98-107
- 全文大小：5252 K
文摘
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A portable temperature-humidity control device was developed for sensitive LFA.
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Quantitative analysis of LFA signal was performed using a smartphone.
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Temperatures of 55-60 °C with a RH > 60% allowed optimum nucleic acid detection in LFA.
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Temperatures of 37-40 °C with a RH > 60% allowed optimum antigen detection in LFA.
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The device holds potential to be coupled with a fully integrated paper-based biosensor.