Isolation of a methanotroph from a hydrogen sulfide-rich anaerobic digester for methanol production from biogas
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Methanotrophs were isolated from a hydrogen sulfide (H2S)-rich anaerobic digester.

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One isolate, Methylocaldum gracile SAD2, can grow at 1000 ppm H2S in the headspace.

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Strain SAD2 showed greater H2S tolerance than two commonly studied methanotrophs.

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Strain SAD2 produced 275 mg/L methanol in the presence of 500 ppm H2S.

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This is the first reported methanotroph that converts H2S-rich biogas to methanol.

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