Different methods have been described to detect the EDNRBIle118Lys mutation, including AS-PCR and PIRA-PCR.

These tests, which have been carried out by polyacrylamide gel electrophoresis (PAGE), are time-consuming techniques.

In our study, we developed a new method based on mutagenically separated PCR (MS-PCR).

Our method provides a fast and simple analysis of the EDNRB horse gene.