Molecular cloning and characterization of a novel basic helix-loop-helix-144 (bHLH144)-like transcription factor from Morus alba (L.)
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- 作者：R.S. Sajeevan ; Karaba N. Nataraja ; nataraja_karaba@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; nnkaraba@uasbagalore.edu.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：bHLH ; Basic helix&ndash ; loop&ndash ; helix ; TF ; Transcription factor ; NCBI ; National Center for Biotechnology Information ; cDNA ; DNA complementary to RNA ; RNA ; Ribonucleic acid ; RACE ; Rapid amplification of cDNA ends ; Ma ; Morus alba ; gDNA ; Genomic DNA ; ORF ; Open reading frame ; BLAST ; Basic Local Alignment Search Tool ; NJ ; Neighbor joining ; MEGA ; Molecular Evolutionary Genetics Analysis ; ExPASy ; Expert Protein Analysis System ; I-TASSER ; Iterative Threading ASSEmbly Refinement ; SIB ; Swiss Institute
- 刊名：Plant Gene
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：5
- 期：Complete
- 页码：109-117
- 全文大小：2497 K
文摘
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Full-length cDNA, transcript variant, and genomic DNA sequences of bHLH144-like gene were cloned from mulberry.
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Fold-Index analysis predicted MabHLH144-like gene is highly disordered protein.
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Differential expression of variants in different tissue types and under abiotic stresses noticed.
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miRNA binding analysis revealed the absence of a target site in one the transcript variants.
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This is the first report on cloning and characterization of transcript variant of bHLH144-like gene from mulberry.