The impact of protein extraction protocols on the performance of currently available MALDI-TOF mass spectrometry for identification of mycobacterial clinical isolates cultured in liquid media
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- 作者：Jeong Su Parka ; b ; Soon Hee Choia ; Sang Mee Hwanga ; b ; Yun Ji Honga ; b ; Taek Soo Kimb ; Kyoung Un Parka ; b ; m91w95pf@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Junghan Songa ; b ; Eui-Chong Kimb
- 关键词：MALDI-TOF mass spectrometry ; Mycobacterium ; Protein extraction ; Liquid culture
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：1 September 2016
- 年：2016
- 卷：460
- 期：Complete
- 页码：190-195
- 全文大小：279 K
文摘
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Protein extraction impacts greatly the performance of MALDI-TOF MS for identification of mycobacterial isolates.
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Protein extraction protocol should be chosen considering the characteristics of MALDI-TOF system.
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Heating-based protein extraction showed 90% accuracy for mycobacterial clinical isolates in Bruker MALDI-TOF system.