Biomechanical profile of cancer stem-like cells derived from MHCC97H cell lines

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• 作者：Jinghui Sun^a ; ^{sunjhemail@163.com" class="auth_mail" title="E-mail the corresponding author} ; Qing Luo^a ; ^{qing.luo@cqu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Lingling Liu^a ; ^{liulingling2012@163.com" class="auth_mail" title="E-mail the corresponding author} ; Bingyu Zhang^a ; ^{zhangbingyu1111@126.com" class="auth_mail" title="E-mail the corresponding author} ; Yisong Shi^a ; ^{shiyis@cqu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yang Ju^b ; ^{ju@mech.nagoya-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Guanbin Song^a ; ^{song@cqu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：HHCs ; human hepatoma cells ; LCSCs ; liver cancer stem-like cells ; AFM ; atomic force microscopy ; SFCs ; sphere-forming cells ; TSM ; tumor sphere medium
• 刊名：Journal of Biomechanics
• 出版年：2016
• 出版时间：4 January 2016
• 年：2016
• 卷：49
• 期：1
• 页码：45-52
• 全文大小：7776 K

文摘

Biomechanical properties and cytoskeletal organization of cancer cells are known to be closely related with their aggressive phenotype. In this study, based on atomic force microscopy (AFM), we aimed to evaluate the mechanical property of liver cancer stem-like cells (LCSCs) and compare it with human hepatoma cells (HHCs). LCSCs were enriched from human hepatoma cell line MHCC97H through a sphere culture system. AFM nanoindentation was investigated as a method for measuring the cell stiffness, and reflecting by Young׳s modulus. Microfilament bundles of F-actin were observed with immunofluorescence staining by confocal microscopy. We found that LCSCs show lower Young׳s modulus and higher migration ability compared to MHCC97H cells. Moreover, the decrease in Young׳s modulus is accompanied with a dramatic decline in F-actin content. These results demonstrated a close relationship between the cell Young׳s modulus and metastatic potential of HHCs, which suggest that Young׳s modulus detected by AFM can be used to evaluate metastatic potential of cancer cells.