Establishment of a highly efficient virus-inducible CRISPR/Cas9 system in insect cells
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- 作者：Zhan-Qi Donga ; Ting-Ting Chena ; Jun Zhanga ; c ; Nan Hua ; Ming-Ya Caoa ; Fei-Fan Donga ; Ya-Ming Jianga ; Peng Chena ; Cheng Lua ; b ; lucheng@swu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Min-Hui Pana ; b ; pmh047@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bombyx mori ; BmNPV ; CRISPR/Cas9 ; Virus-inducible CRISPR/Cas9 system ; Antiviral
- 刊名：Antiviral Research
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：130
- 期：Complete
- 页码：50-57
- 全文大小：1591 K
文摘
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CRISPR/Cas9 system is capable of specifically disrupting ie-1 gene in BmNPV genome.
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The CRISPR/Cas9 system effectively edits BmNPV genome and inhibits virus replication.
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Virus-inducible CRISPR/Cas9 system is expected to reduce the probability of off-target effects in the insect cells.