Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells
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- 作者：Zhongliang Liu1 ; 6 ; Yi Hui2 ; 6 ; Lei Shi3 ; 6 ; Zhenyu Chen1 ; Xiangjie Xu1 ; Liankai Chi1 ; Beibei Fan1 ; Yujiang Fang1 ; Yang Liu1 ; Lin Ma1 ; Yiran Wang1 ; Lei Xiao3 ; Quanbin Zhang1 ; Guohua Jin2 ; jguohua@ntu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Ling Liu1 ; 4 ; lliu@tongji.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaoqing Zhang1 ; 4 ; 5 ; xqzhang@tongji.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：CRISPR/Cas9 ; human pluripotent stem cells ; gene editing ; non-coding RNA
- 刊名：Stem Cell Reports
- 出版年：2016
- 出版时间：13 September 2016
- 年：2016
- 卷：7
- 期：3
- 页码：496-507
- 全文大小：2869 K
文摘
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Paired-KO represents an efficient gene ablation methodology in hPSCs
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The cleavage sites and ligation pattern can be easily predicted in paired-KO
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Paired-KO fits for loss-of-function studies for both coding and non-coding genes
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Promoter removal is sufficient to eradicate downstream gene transcription