The effects of subsampling gene trees on coalescent methods applied to ancient divergences
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Pairwise congruence among gene trees varied greatly between and within studies.

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ASTRAL-based coalescent analyses generally outperformed MP-EST and STAR.

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Subsampling most congruent gene trees may reduce gene-tree-error artifacts.

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We present a script to automate the subsampling process.

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Multiple tree-search replicates should be implemented for MP-EST analyses.

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