The recombiant neutral protease was expressed in Pichia pastoris at a high level under high-density fermentation.
The gene of recombiant neutral protease was modified based on the codon usage bias of P. Pastoris.
Low concentrations of zinc, calcium and magnesium ions stimulated the recombiant neutral protease enzyme activity.
All of the evidence indicated that the recombiant neutral protease is a thermolysin-like peptidase.