Imaging viral RNA using multiply labeled tetravalent RNA imaging probes in live cells
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- 作者：Eric Alonas ; Daryll Vanover ; Emmeline Blanchard ; Chiara Zurla ; Philip J. Santangelo ; philip.santangelo@bme.gatech.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Viral RNA ; Fluorescence ; Live cell imaging
- 刊名：Methods
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：98
- 期：Complete
- 页码：91-98
- 全文大小：945 K
文摘
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Reviews RNA imaging techniques including FISH, MS2-GFP, Spinach, and MTRIPs.
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Protocols are provided for using MTRIPs to label respiratory syncytial virus (RSV).
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Illustrates how to use labeled virus for single virion and single cell imaging.