Development of an automated mid-scale parallel protein purification system for antibody purification and affinity chromatography
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- 作者：Chi Zhangb ; Alexander M. Longa ; Brooke Swalma ; Ken Charesta ; Yan Wanga ; Jiali Hua ; Craig Schulza ; Wolfgang Goetzingera ; Brian E. Halla ; brianhall22@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Automation ; High-throughput protein purification ; Affinity chromatography ; Monoclonal antibody (mAb)
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：29-35
- 全文大小：1566 K
文摘
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Using a modified Solid Phase Extraction System an automated mid-scale protein purification system was developed.
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24 samples of up to 50 ml volume can be purified by affinity chromatography overnight.
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mAbs were purified to compare the SPE system with an industry leading AKTA system.