Quercetin affects glutathione levels and redox ratio in human aortic endothelial cells not through oxidation but formation and cellular export of quercetin-glutathione conjugates and upregulation of glutamate-cysteine ligase
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- 作者：Chuan Li ; Wei-Jian Zhang ; Jaewoo Choi ; Balz Frei ; balz.frei@oregonstate.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：AAP ; ascorbic acid phosphate ; BSO ; buthionine sulfoximine ; BPDS ; bathophenanthrolinedisulfonic acid ; DCFH-DA ; 2&prime ; 7&prime ; -dichlorofluorescein diacetate ; GCL ; glutamate-cysteine ligase ; GCLC ; catalytic subunit of glutamate-cysteine ligase ; GCLM ; modifier subunit of glutamate-cysteine ligase ; GSH ; glutathione ; GSSG ; glutathione disulfide ; HAEC ; human aortic endothelial cells ; IAA ; iodoacetic acid ; LC-MS ; liquid chromatography-mass spectrometry ; LPS ; lipopolysaccharide ; MRM ; multiple reaction
- 刊名：Redox Biology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：9
- 期：Complete
- 页码：220-228
- 全文大小：831 K
文摘
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Glutathione mediates the antioxidant effects of quercetin in human aortic endothelial cells.
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Quercetin affects cellular levels of GSH and GSSG, resulting in an increased redox ratio.
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Quercetin forms conjugates with GSH, which are rapidly excreted from the cells.
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Quercetin induces glutamate-cysteine ligase and multidrug resistance protein 1 via Nrf2 activation.