Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology

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• 作者：Dandan Zhang ; Zhenxiang Li ; Jian-Feng Li ; ^{lijfeng3@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CRISPR/Cas technology ; Genome editing ; Gene knockout ; Plants
• 刊名：Journal of Genetics and Genomics
• 出版年：2016
• 出版时间：20 May 2016
• 年：2016
• 卷：43
• 期：5
• 页码：251-262
• 全文大小：601 K

文摘

The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse organisms including plants, and has quickly evolved into a suite of versatile tools for sequence-specific gene manipulations beyond genome editing. Here, we review the most recent applications of the CRISPR/Cas toolkit in plants and also discuss key factors for improving CRISPR/Cas performance and strategies for reducing the off-target effects. Novel technical breakthroughs in mammalian research regarding the CRISPR/Cas toolkit will also be incorporated into this review in hope to stimulate prospective users from the plant research community to fully explore the potential of these technologies.