S-Sulfocysteine simplifies fed-batch processes and increases the CHO specific productivity via anti-oxidant activity
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- 作者：Caroline Hecklaua ; Caroline.Hecklau@external.merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Sascha Peringa ; Sascha.Pering@external.merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Ronja Seibela ; Ronja.Seibel@merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Alisa Schnellbaechera ; Alisa.Schnellbaecher@merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Maria Wehslinga ; Maria.Wehsling@merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Thomas Eichhornb ; Thomas.Eichhorn@merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Joerg von Hagena ; Joerg.von.Hagen@merckgroup.com" class="auth_mail" title="E-mail the corresponding author ; Aline Zimmera ; Aline.Zimmer@merckgroup.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cysteine stability ; S-Sulfocysteine ; Fed-batch ; Monoclonal antibody ; Productivity ; Single feed
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：218
- 期：Complete
- 页码：53-63
- 全文大小：2560 K
文摘
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Fed-batch processes can be simplified by using S-sulfocysteine as a cysteine source.
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S-sulfocysteine is stable in neutral pH feeds for months.
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S-sulfocysteine increases CHO specific productivity via antioxidant activity.
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The modified amino acid is not integrated in the produced monoclonal antibody.