A collaborative study of an alternative in vitro potency assay for the Japanese encephalitis vaccine
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- 作者：Byung-Chul Kima ; b ; 1 ; Do-Keun Kima ; c ; 1 ; Hyung-Jin Kima ; Seung-Hwa Honga ; Yeonhee Kima ; Jong-Mi Lima ; JiYoung Honga ; Cheol-Hee Kimb ; Yong-Keun Parkc ; Jaeok Kima ; kimjo70@korea.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：JE ; Japanese encephalitis ; PRN ; plaque reduction neutralization ; ELISA ; enzyme-linked immunosorbent assay ; CEC ; chick embryo cell ; EMEM ; Eagle's minimum essential medium ; CV ; coefficient of variation
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 September 2016
- 年：2016
- 卷：223
- 期：Complete
- 页码：190-196
- 全文大小：1365 K
文摘
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We developed an alternative in vitro ELISA to determine the E antigen content of the Japanese encephalitis virus to observe the 3Rs strategy.
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Our study demonstrated that an in vitro assay could perform faster and was more convenient than the established in vivo PRNTest for JEV vaccine.
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Thus, we propose a progressive switch from the in vivo assay to the in vitro ELISA for JEV vaccine quality control.