Optimization of keratin/alginate scaffold using RSM and its characterization for tissue engineering

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• 作者：Pratima Gupta ; ^{pguptabiotech@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{pratibiotech@yahoo.co.in" class="auth_mail" title="E-mail the corresponding author} ; Kush Kumar Nayak ^{nayak23bt@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：RSM ; Scaffold fabrication ; Mechanical behavior ; Porosity ; Structural characterization ; Antimicrobial activity
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：85
• 期：Complete
• 页码：141-149
• 全文大小：2356 K

文摘

The scaffold for tissue engineering was fabricated from a binary blend of keratin/alginate. The concentration and ratio of keratin and alginate was optimized by response surface methodology in a scaffold. The structural compatibility between keratin and alginate was examined by X-ray diffractometer and Fourier transforms infrared spectroscopy. Apparent porosity of the scaffold was calculated by Archimedes principles and its observed value of was found 96.25 ± 0.04%. The pore size of the scaffold was observed in the range between 10 and 200 μm. Tensile strength (0.33 ± 0.26 MPa) and percent of elongation at break (23.33 ± 2.52%) are the reported mechanical strength of the scaffold. Positive antimicrobial activity and in vitro degradation further confirms the fabrication of a scaffold required for tissue engineering application.