Sequence-non-specific effects generated by various types of RNA interference triggers
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- 作者：Marta Olejniczaka ; Marta.olejniczak@ibch.poznan.pl" class="auth_mail" title="E-mail the corresponding author ; Martyna O. Urbaneka ; Martyna.urbanek@ibch.poznan.pl" class="auth_mail" title="E-mail the corresponding author ; Edyta Jaworskaa ; edyjaw@ibch.poznan.pl" class="auth_mail" title="E-mail the corresponding author ; Lukasz Wituckia ; lukwitucki@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Michal W. Szczesniakb ; miszcz@amu.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Izabela Makalowskab ; izabel@amu.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Wlodzimierz J. Krzyzosiaka ; wlodkrzy@ibch.poznan.pl" class="auth_mail" title="E-mail the corresponding author
- 关键词：RNA interference ; siRNA ; Immune response ; miRNA ; Off-target ; isomiRs ; RNAimmuno
- 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：1859
- 期：2
- 页码：306-314
- 全文大小：1363 K
文摘
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vector-based RNAi triggers are stronger stimulators of IFN response than siRNAs.
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most of the effects induced by vector-based reagents result from the transfection of plasmid DNA.
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TLR3 and IFNα2 as specific molecular markers of immune response stimulation by siRNA and shRNA/sh-miRs, respectively.
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poly(I:C) may not serve as a universal positive control of immune response stimulation in RNAi experiments.
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transfection of RNAi triggers influences miRNA levels and the isomiRs composition