In vitro evaluation of traditionally used Surinamese medicinal plants for their potential anti-leishmanial efficacy

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• 作者：D.R.A. Mans^a ; ^{dennis_mans@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; ^{dennis.mans@uvs.edu" class="auth_mail" title="E-mail the corresponding author} ; T. Beerens^b ; I. Magali^a ; R.C. Soekhoe^a ; G.J. Schoone^b ; K. Oedairadjsingh^a ; J.A. Hasrat^a ; E. van den Bogaart^b ; H.D.F.H. Schallig^b
• 关键词：leishmaniasis ; Suriname ; medicinal plants ; promastigote assay ; amastigote-phagocyte assay ; anti-leishmanial activity
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：2 March 2016
• 年：2016
• 卷：180
• 期：Complete
• 页码：70-77
• 全文大小：336 K

文摘

Plant-based preparations are extensively used in Surinamese folk medicine for treating leishmaniasis, but often without a scientific rationale.

Aim of the study

To evaluate 25 Surinamese medicinal plants for their potential efficacy against leishmaniasis.

Materials and methods

Concentrated plant extracts were evaluated for their effect on the viability of L. (V.) guyanensis AMC, L. (L.) major NADIM5, and L. (L.) donovani GEDII promastigotes, as well as intracellular amastigotes of L. (L.) donovani BHU814 in infected THP-1 cells. Selectivity was assessed by cytotoxicity against THP-1 cells.

Results

The only plant extract that showed potentially meaningful anti-leishmanial activity was that from Solanum lycocarpum that displayed mean IC₅₀ values of about 51, 61, and <16 µg/mL against L. (V) guyanensis, L. (L) major, and L. (L) donovani promastigotes, respectively; about 374 µg/mL against L. (L) donovani amastigotes; and >500 µg/mL against THP-1 cells. The Bryophyllum pinnatum, Inga alba, and Quassia amara extracts displayed moderate to high IC₅₀ values against promastigotes (about 51 to >500 µg/mL) and/or amastigotes (about 224 to >500 µg/mL) but were relatively toxic to THP-1 cells (IC₅₀ values <16 to about 42 µg/mL). The remaining plant extracts exhibited in many cases IC₅₀ values close to, around, or above 500 µg/mL against promastigotes, amastigotes, and THP-1 cells.

Conclusions

The S. lycocarpum preparation may be useful against leishmaniasis and may have a good safety index, warranting further investigations into its active constituents and mechanism(s) of action.