DNA sequencing confirms PCR-RFLP identification of wild caught Larroussius sand flies from Crete and Cyprus
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- 作者：Emmanouil Dokianakisa ; m.dokianakis@med.uoc.gr" class="auth_mail" title="E-mail the corresponding author ; Nikolaos Tsirigotakisa ; n.tsirigotakis@med.uoc.gr" class="auth_mail" title="E-mail the corresponding author ; Vasiliki Christodouloub ; vchristod@edu.med.uoc.gr" class="auth_mail" title="E-mail the corresponding author ; Nikos Poulakakisc ; d ; poulakakis@nhmc.uoc.gr" class="auth_mail" title="E-mail the corresponding author ; Maria Antonioua ; antoniou@uoc.gr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sand fly ; Phlebotomus ; Leishmaniasis ; PCR &ndash ; RFLP ; DNA barcoding ; COI ; Larroussius ; Crete ; Cyprus ; Systematics
- 刊名：Acta Tropica
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：164
- 期：Complete
- 页码：314-320
- 全文大小：1307 K
文摘
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The Larroussius subgenus contains sand fly species which are important vectors of the protozoan parasite Leishmania.
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Morphological identification, especially of females, is difficult and may pose obstacles during epidemiological surveys.
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The identification of wild caught Larroussius sand flies, using a combination of morphology and COI PCR-RFLP, is presented.
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Molecular Identification results were confirmed by DNA barcoding and Bayesian phylogenetic analysis.