Tick iron and heme metabolism - New target for an anti-tick intervention

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• 作者：Ondrej Hajdusek ; ^{hajdus@paru.cas.cz" class="auth_mail" title="E-mail the corresponding author} ; Radek Sima ^{sima@paru.cas.cz" class="auth_mail" title="E-mail the corresponding author} ; Jan Perner ^{perner@paru.cas.cz" class="auth_mail" title="E-mail the corresponding author} ; Gabriela Loosova ^{gabi@paru.cas.cz" class="auth_mail" title="E-mail the corresponding author} ; Adela Harcubova ^{harcubova@paru.cas.cz" class="auth_mail" title="E-mail the corresponding author} ; Petr Kopacek ^{kopajz@paru.cas.cz" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tick ; Iron ; Heme ; RNAi ; Vaccine
• 刊名：Ticks and Tick-borne Diseases
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：7
• 期：4
• 页码：565-572
• 全文大小：1440 K

文摘

Ticks are blood-feeding parasites and vectors of serious human and animal diseases. Ixodes ricinus is a common tick in Europe, transmitting tick-borne encephalitis, Lyme borreliosis, anaplasmosis, or babesiosis. Immunization of hosts with recombinant tick proteins has, in theory, the potential to interfere with tick feeding and block transmission of pathogens from the tick to the host. However, the efficacy of tick antigens has, to date, not been fully sufficient to achieve this. We have focused on 11 in silico identified genes encoding proteins potentially involved in tick iron and heme metabolism. Quantitative real-time PCR (qRT-PCR) expression profiling was carried out to preferentially target proteins that are up-regulated during the blood meal. RNA interference (RNAi) was then used to score the relative importance of these genes in tick physiology. Finally, we performed vaccination screens to test the suitability of these proteins as vaccine candidates. These newly identified tick antigens have the potential to improve the available anti-tick vaccines.