Genome Editing: From Drosophila to Non-Model Insects and Beyond

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• 作者：Yueping Huang ; Zhiping Liu ; Yikang S. Rong ; ^{zdqr03@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; ^{rongyik@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Insect transgenesis ; Drosophila ; Black soldier fly ; Gene targeting ; Site-specific recombination ; Piggybac transposon
• 刊名：Journal of Genetics and Genomics
• 出版年：2016
• 出版时间：20 May 2016
• 年：2016
• 卷：43
• 期：5
• 页码：263-272
• 全文大小：1084 K

文摘

Insect is the largest group of animals on land. Many insect species inflict economical and health losses to humans. Yet many more benefit us by helping to maintain balances in our ecosystem. The benefits that insects offer remain largely untapped, justifying our continuing efforts to develop tools to better understand their biology and to better manage their activities. Here we focus on reviewing the progresses made in the development of genome engineering tools for model insects. Instead of detailed descriptions of the molecular mechanisms underlying each technical advance, we focus our discussion on the logistics for implementing similar tools in non-model insects. Since none of the tools were developed specific for insects, similar approaches can be applied to other non-model organisms.