Real-time and visual loop-mediated isothermal amplification: Efficient GMO screening targeting pat and pmi marker genes
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- 作者：Monika Singh monika_a_singh@rediffmail.com" class="auth_mail" title="E-mail the corresponding author monika.singh@icar.gov.in" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Rajesh K. Bhoge rajbhoge09@yahoo.in" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Gurinderjit Randhawa ; gurinder.randhawa@rediffmail.com" class="auth_mail" title="E-mail the corresponding author ; gurinder.randhawa@icar.gov.in" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：GMO screening ; LAMP ; Marker genes ; On-site detection ; pat ; pmi
- 刊名：Food Control
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：71
- 期：Complete
- 页码：248-254
- 全文大小：1286 K
文摘
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Visual and real-time loop-mediated isothermal amplification (LAMP) assays targeting pat and pmi were developed.
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LAMP assays for pmi gene have been reported for the first time.
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Limit of detection (LOD) of visual LAMP was up to 0.05% genetically modified (GM) content for pat and 0.1% for pmi.
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Real-time LAMP efficiently detected up to 0.01% GM content within 30 min.
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These assays could be employed to screen 35% of GM events approved globally.