Kinetic investigations of 6-phosphogluconate dehydrogenase confined in mesoporous silica
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- 作者：Michael Dreifke ; Dorothee I. Fried ; Felix J. Brieler ; Michael Frö ; ba ; froeba@chemie.uni-hamburg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mesoporous cellular siliceous foams ; Surface functionalization ; 6-phosphogluconate dehydrogenase ; Enzyme immobilization ; Michaelis-Menten kinetic
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：132
- 期：Complete
- 页码：5-15
- 全文大小：1680 K
文摘
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Immobilization of G.s.6PGDH onto alkyl and aminoalkyl modified MCFs is possible.
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Immobilization is initiated due to hydrophobic interactions.
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Activity retention is highest when employing a 0.5 mg mL−1 6PGDH solution for immobilization.
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The stability of the immobilized 6PGDH shows a dependency on the spacer length.
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Investigation of the Michaelis-Menten kinetics proposed a C5-chain as optimal spacer.