High vitamin A intake during pregnancy modifies dopaminergic reward system and decreases preference for sucrose in Wistar rat offspring

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• 作者：Diana Sá ; nchez-Herná ; ndez^a ; ^{diana.sanchezhernandez@mail.utoronto.ca" class="auth_mail" title="E-mail the corresponding author} ; Abraham N. Poon^a ; ^{abepoon87@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Ruslan Kubant^a ; ^{ruslan.kubant@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Hwanki Kim^a ; ^{brianhk.kim@mail.utoronto.ca" class="auth_mail" title="E-mail the corresponding author} ; Pedro S.P. Huot^a ; ^{pedro.huot@mail.utoronto.ca" class="auth_mail" title="E-mail the corresponding author} ; Clara E. Cho^a ; ¹ ; ^{cec297@cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Emanuela Pannia^a ; ^{e.pannia@mail.utoronto.ca" class="auth_mail" title="E-mail the corresponding author} ; Sandra A. Reza-Ló ; pez^a ; ² ; ^{sandyreza01@yahoo.com.mx" class="auth_mail" title="E-mail the corresponding author} ; Zdenka Pausova^a ; ^c ; ^{zdenka.pausova@sickkids.ca" class="auth_mail" title="E-mail the corresponding author} ; Richard P. Bazinet^a ; ^{richard.bazinet@utoronto.ca" class="auth_mail" title="E-mail the corresponding author} ; G. Harvey Anderson^a ; ^b ; ^{harvey.anderson@utoronto.ca" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Comt ; Catechol-O-methyl transferase ; CpG ; Cytosine-phosphate-guanine ; Darpp-32 ; DA- and cyclic AMP-regulated phosphoprotein ; Dat ; Dopamine transporter ; Drd1 ; Dopamine receptor 1 ; Drd2 ; Dopamine receptor 2 ; Drd5 ; Dopamine receptor 5 ; HA ; AIN-93G diet with 10-fold the amount of vitamin A ; HV ; AIN-93G diet with 10-fold multivitamin levels ; HVRA ; AIN-93G diet with 10-fold multivitamins levels ; 1-fold vitamin A ; Npy ; Neuropeptide Y ; Pomc ; Proopiomelanocortin ; RARE ; Retinoic acid response element ; RV
• 刊名：Journal of Nutritional Biochemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：27
• 期：Complete
• 页码：104-111
• 全文大小：465 K

文摘

High multivitamin (HV) content in gestational diets has long-term metabolic effects in rat offspring. These changes are associated with in utero modifications of gene expression in hypothalamic food intake regulation. However, the role of fat-soluble vitamins in mediating these effects has not been explored. Vitamin A is a plausible candidate due to its role in gene methylation. Vitamin A intake above requirements during pregnancy affects the development of neurocircuitries involved in food intake and reward regulation. Pregnant Wistar rats were fed AIN-93G diets with the following content: recommended multivitamins (1-fold multivitamins: RV), high vitamin A (10-fold vitamin A: HA) or HV with only recommended vitamin A (10-fold multivitamins, 1-fold vitamin A: HVRA). Body weight, food intake and preference, mRNA expression and DNA methylation of hippocampal dopamine-related genes were assessed in male offspring brains at different developmental windows: birth, weaning and 14 weeks postweaning. HA offspring had changes in dopamine-related gene expression at all developmental windows and DNA hypermethylation in the dopamine receptor 2 promoter region compared to RV offspring. Furthermore, HA diet lowered sucrose preference but had no effect on body weight and expression of hypothalamic genes. In contrast, HVRA offspring showed only at adulthood changes in expression of hippocampal genes and a modest effect on hypothalamic genes. High vitamin A intake alone in gestational diets has long-lasting programming effects on the dopaminergic system that are further translated into decreased sucrose preference but not food intake.