The influence of abscisic acid on the ethylene biosynthesis pathway in the functioning of the flower abscission zone in Lupinus luteus

详细信息    查看全文

• 作者：Emilia Wilmowicz^a ; ^b ; ^{emwil@umk.pl" class="auth_mail" title="E-mail the corresponding author} ; Kamil Frankowski^a ; ^{kfrank@o2.pl" class="auth_mail" title="E-mail the corresponding author} ; Agata Kućko^a ; ^{kuckoa@poczta.onet.pl" class="auth_mail" title="E-mail the corresponding author} ; Michał Świdziński^c ; ^{shihan@poczta.onet.pl" class="auth_mail" title="E-mail the corresponding author} ; Juan de Dios Alché ; ^d ; ^{juandedios.alche@eez.csic.es" class="auth_mail" title="E-mail the corresponding author} ; Anna Nowakowska^e ; ^{noann@umk.pl" class="auth_mail" title="E-mail the corresponding author} ; Jan Kopcewicz^a ; ^{kopcew@umk.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ABA ; abscisic acid ; ACC ; 1-aminocyclopropane-1-carboxylic acid ; ACS ; ACC synthase gene ; ACO ; ACC oxidase gene ; AZ ; abscission zone ; ET ; ethylene ; NBD ; 2 ; 5-norbornadiene ; NDGA ; nordihydroguaiaretic acid
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 November 2016
• 年：2016
• 卷：206
• 期：Complete
• 页码：49-58
• 全文大小：3031 K

文摘

Flower abscission is a highly regulated developmental process activated in response to exogenous (e.g. changing environmental conditions) and endogenous stimuli (e.g. phytohormones). Ethylene (ET) and abscisic acid (ABA) are very effective stimulators of flower abortion in Lupinus luteus, which is a widely cultivated species in Poland, Australia and Mediterranean countries. In this paper, we show that artificial activation of abscission by flower removal caused an accumulation of ABA in the abscission zone (AZ). Moreover, the blocking of that phytohormone’s biosynthesis by NDGA (nordihydroguaiaretic acid) decreased the number of abscised flowers. However, the application of NBD – an inhibitor of ET action – reversed the stimulatory effect of ABA on flower abscission, indicating that ABA itself is not sufficient to turn on the organ separation. Our analysis revealed that exogenous ABA significantly accelerated the transcriptional activity of the ET biosynthesis genes ACC synthase (LlACS) and oxidase (LlACO), and moreover, strongly increased the level of 1-aminocyclopropane-1-carboxylic acid (ACC) – ET precursor, which was specifically localized within AZ cells. We cannot exclude the possibility that ABA mediates flower abscission processes by enhancing the ET biosynthesis rate. The findings of our study will contribute to the overall basic knowledge on the phytohormone-regulated generative organs abscission in L. luteus.