Reliable and versatile immortal muscle cell models from healthy and myotonic dystrophy type 1 primary human myoblasts
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- 作者：Boris Pantica ; boris.pantic@yahoo.it" class="auth_mail" title="E-mail the corresponding author ; Doriana Borgiaa ; doriana89b@hotmail.it" class="auth_mail" title="E-mail the corresponding author ; Silvia Giuncob ; silvia.giunco@unipd.it" class="auth_mail" title="E-mail the corresponding author ; Adriana Malenaa ; adriana.malena@unipd.it" class="auth_mail" title="E-mail the corresponding author ; Tohru Kiyonoc ; tkiyono@ncc.go.jp" class="auth_mail" title="E-mail the corresponding author ; Sergio Salvatorid ; patgen08@mail.bio.unipd.it" class="auth_mail" title="E-mail the corresponding author ; Anita De Rossib ; e ; anita.derossi@unipd.it" class="auth_mail" title="E-mail the corresponding author ; Emiliano Giardinaf ; g ; giardina@med.uniroma2.it" class="auth_mail" title="E-mail the corresponding author ; Federica Sangiuolof ; sangiuolo@med.uniroma2.it" class="auth_mail" title="E-mail the corresponding author ; Elena Pegoraroa ; elena.pegoraro@unipd.it" class="auth_mail" title="E-mail the corresponding author ; Lodovica Vergania ; lodovica.vergani@unipd.it" class="auth_mail" title="E-mail the corresponding author ; Annalisa Bottaf ; botta@med.uniroma2.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Human muscle cell models ; Immortalization ; Myotonic dystrophy type 1 ; Cyclin D1 ; TERT ; CDK4 ; ASOs
- 刊名：Experimental Cell Research
- 出版年：2016
- 出版时间：1 March 2016
- 年：2016
- 卷：342
- 期：1
- 页码：39-51
- 全文大小：6951 K
文摘
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CCND1, CDK4 and TERT efficiently immortalize primary human muscle cells
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Healthy and DM1 immortal cells efficiently differentiate after prolonged cell culture
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Immortalized cells do not acquire the transformed phenotype in vitro
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Immortal DM1 muscle cells maintain the molecular features of the disease