Liquid phase separation methods for N-glycosylation analysis of glycoproteins of biomedical and biopharmaceutical interest. A critical review

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• 作者：Laszlo Hajba^aAuthor Vitae ; Eszter Csanky^bAuthor Vitae ; Andras Guttman^a ; ^c ; ^{guttman@mik.uni-pannon.hu" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Recombinant protein therapeutics ; Serum glycoproteins ; N-glycans ; Liquid phase separations ; Fluorescent labels
• 刊名：Analytica Chimica Acta
• 出版年：2016
• 出版时间：2 November 2016
• 年：2016
• 卷：943
• 期：Complete
• 页码：8-16
• 全文大小：958 K

文摘

Comprehensive carbohydrate analysis of glycoproteins from human biological samples and biotherapeutics are important from diagnostic and therapeutic points of view. This review summarizes the current state-of-the-art liquid phase separation techniques used in N-glycosylation analysis. The different liquid chromatographic techniques and capillary electrophoresis methods are critically discussed in detail. Miniaturization of these methods is also important to increase throughput and decrease analysis time. The sample preparation and labeling methods for asparagine linked oligosaccharides are also addressed.