Enhanced chemiluminescence-based detection on gold substrate after electrografting of diazonium precursor-coated gold nanoparticles

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• 作者：Ali Houmed Adabo^a ; ^{alialfred@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Rabah Zeggari^b ; ^{rabah.zeggari@femto-st.fr" class="auth_mail" title="E-mail the corresponding author} ; Nasser Mohamed Saï ; d^a ; ^{said.nasser_mohamed@univ-fcomte.fr" class="auth_mail" title="E-mail the corresponding author} ; Rana Bazzi^a ; ^{rana.bazzi@univ-fcomte.fr" class="auth_mail" title="E-mail the corresponding author} ; Cé ; line Elie-Caille^b ; ^{celine.caille@femto-st.fr" class="auth_mail" title="E-mail the corresponding author} ; Christophe Marquette^c ; ^{christophe.marquette@univ-lyon1.fr" class="auth_mail" title="E-mail the corresponding author} ; Matteo Martini^d ; ^{matteo.martini@univ-lyon1.fr" class="auth_mail" title="E-mail the corresponding author} ; Olivier Tillement^d ; ^{olivier.tillement@univ-lyon1.fr" class="auth_mail" title="E-mail the corresponding author} ; Pascal Perriat^e ; ^{pascal.perriat@insa-lyon.fr" class="auth_mail" title="E-mail the corresponding author} ; Carole Chaix^f ; ^{carole.chaix-bauvais@univ-lyon1.fr" class="auth_mail" title="E-mail the corresponding author} ; Wilfrid Boireau^b ; ^{wilfrid.boireau@femto-st.fr" class="auth_mail" title="E-mail the corresponding author} ; Sté ; phane Roux^a ; ^{stephane.roux@univ-fcomte.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AFM ; Atomic Force Microscopy ; a.u. ; arbitrary units ; Au@MA ; mercaptoaniline-coated gold nanoparticles ; CCD ; charge-coupled device ; GC ; glassy carbon ; HClaq ; hydrochloric acid ; ICP-MS ; Inductively Coupled Plasma-Mass Spectrometry ; ITO ; indium tin oxide ; MA ; mercaptoaniline ; MWCO ; molecular weight cut-off ; P5 ; tri-thiolated pentapeptide modified with a biotin molecule at its N-terminal end ; PBS ; Phosphate Buffer Saline ; SAP ; streptavidin&ndash ; peroxidase ; SCE ; saturated calomel electrode ; TEM ; Tra
• 刊名：Journal of Colloid and Interface Science
• 出版年：2016
• 出版时间：1 April 2016
• 年：2016
• 卷：467
• 期：Complete
• 页码：271-279
• 全文大小：1977 K

文摘

Since it was demonstrated that nanostructured surfaces are more efficient for the detection based on the specific capture of analytes, there is a real need to develop strategies for grafting nanoparticles onto flat surfaces. Among the different routes for the functionalization of a surface, the reduction of diazonium salts appears very attractive for the covalent immobilization of nanoparticles because this method does not require a pre-treatment of the surface. For achieving this goal, gold nanoparticles coated by precursor of diazonium salts were synthesized by reduction of gold salt in presence of mercaptoaniline. These mercaptoaniline-coated gold nanoparticles (Au@MA) were successfully immobilized onto various conducting substrates (indium tin oxide (ITO), glassy carbon (GC) and gold electrodes with flat terraces) after addition of sodium nitrite at fixed potential. When applied onto the gold electrodes, such a grafting strategy led to an obvious enhancement of the luminescence of luminol used for the biodetection.