Employing bacterial microcompartment technology to engineer a shell-free enzyme-aggregate for enhanced 1,2-propanediol production in Escherichia coli
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- 作者：Matthew J. Leea ; Ian R. Browna ; Rokas Juodeikisa ; Stefanie Franka ; S.Frank@kent.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Martin J. Warrena ; m.j.warren@kent.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：BMC ; bacterial microcompartment ; Pdu ; 1 ; 2-propanediol utilisation ; 1 ; 2-PD ; 1 ; 2-propanediol ; D18 ; First 18 amino acids of PduD ; P18 ; First 18 amino acids of PduP
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：36
- 期：Complete
- 页码：48-56
- 全文大小：2629 K
文摘
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Fusion of BMC targeting peptides to enzymes has a variable effect on activity.
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Tagged enzymes for 1,2-propanediol synthesis are localised to a BMC.
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BMC-targeted proteins localised within the BMC are protected from proteases.
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TEM reveals tagged enzymes form large intracellular protein aggregates.
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Strains with enzyme aggregates are shown to have enhanced 1,2-propanediol production.