Response surface analysis was used for optimizing amylose (AM) aqueous leaching.
Starch concentration (SC) and leaching temperature (LT) influence AM leaching.
SC has a significant effect which depends on LT.
Leaching of long AM chains increases with LT but at the expense of AM purity.
High yield and purity of long AM chains are obtained at 81 °C (LT) and 3.3% (SC).