Infusion of solutions of pre-irradiated components in rats

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• 作者：Georgina Pappas^a ; ^{Georgina.Pappas@quinnipiac.edu" class="auth_mail" title="E-mail the corresponding author} ; Francoise Arnaud^a ; ^b ; ^{francoise.arnaud.ctr@mail.mil" class="auth_mail" title="E-mail the corresponding author} ; Ashraful Haque^a ; ^{ashraful.haque.ctr@mail.mil" class="auth_mail" title="E-mail the corresponding author} ; Tomoyuki Kino^a ; ^{tmykkn1974@yahoo.co.jp" class="auth_mail" title="E-mail the corresponding author} ; Paul Facemire^c ; ^{paul.r.facemire2.mil@mail.mil" class="auth_mail" title="E-mail the corresponding author} ; Erica Carroll^c ; ^{erica.e.carroll.mil@mail.mil" class="auth_mail" title="E-mail the corresponding author} ; Charles Auker^a ; ^{charles.r.auker.ctr@mail.mil" class="auth_mail" title="E-mail the corresponding author} ; Richard McCarron^a ; ^b ; ^{richard.m.mccarron.cib@mail.mil" class="auth_mail" title="E-mail the corresponding author} ; Anke Scultetus^a ; ^b ; ^{anke.h.scultetus2.ctr@mail.mil" class="auth_mail" title="E-mail the corresponding author}
• 关键词：FIVR ; field intravenous resuscitation
• 刊名：Experimental and Toxicologic Pathology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：68
• 期：6
• 页码：355-363
• 全文大小：494 K

文摘

The objective of this study was to conduct a 14-day toxicology assessment for intravenous solutions prepared from irradiated resuscitation fluid components and sterile water.

Methods

Healthy Sprague Dawley rats (7–10/group) were instrumented and randomized to receive one of the following Field IntraVenous Resuscitation (FIVR) or commercial fluids; Normal Saline (NS), Lactated Ringer’s, 5% Dextrose in NS. Daily clinical observation, chemistry and hematology on days 1,7,14, and urinalysis on day 14 were evaluated for equivalence using a two sample t-test (p<0.05). A board-certified pathologist evaluated organ histopathology on day 14.

Results

Equivalence was established for all observation parameters, lactate, sodium, liver enzymes, creatinine, WBC and differential, and urinalysis values. Lack of equivalence for hemoglobin (p = 0.055), pH (p = 0.0955), glucose (p = 0.0889), Alanine-Aminotransferase (p = 0.1938), albumin (p = 0.1311), and weight (p = 0.0555, p = 0.1896), was deemed not clinically relevant due to means within physiologically normal ranges. Common microscopic findings randomly distributed among animals of all groups were endocarditis/myocarditis and pulmonary lesions.

Discussion

These findings are consistent with complications due to long-term catheter use and suggest no clinically relevant differences in end-organ toxicity between animals infused with FIVR versus commercial fluids.