Going native: Complete removal of protein purification affinity tags by simple modification of existing tags and proteases
详细信息 查看全文
- 作者：Hui Chin Goha ; Radoslaw M. Sobotab ; Farid J. Ghadessya ; fghadessy@p53Lab.a-star.edu.sg" class="auth_mail" title="E-mail the corresponding author ; Saurabh Nirantara ; saurabh.nirantar79@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein purification ; Affinity tags ; Protease ; Enforced colocalisation ; Native protein
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：18-24
- 全文大小：1389 K
文摘
- class="listitem" id="list_ulist0010">
- class="label">•
A method developed to improve protease cleavage of recalcitrant fusion proteins to yield completely tag-free proteins.
- class="label">•
SplB and TEV proteases adapted for improved cleavage.
- class="label">•
On-column cleavage of histidine tagged fusion protein demonstrated.
- class="label">•
Native status verified by mass spectrophotometry analysis.