Selection of fluorescent DNA dyes for real-time LAMP with portable and simple optics
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- 作者：Gregoire Seyriga ; Robert D. Stedtfelda ; Dieter M. Tourloussea ; Farhan Ahmada ; Keara Towerya ; Alison M. Cupplesa ; James M. Tiedjeb ; c ; Syed A. Hashshama ; b ; hashsham@egr.msu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Loop-mediated isothermal amplification ; DNA dyes ; Syto ; Point of care
- 刊名：Journal of Microbiological Methods
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：119
- 期：Complete
- 页码：223-227
- 全文大小：561 K
文摘
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Eleven DNA dyes were compared for real-time loop-mediated isothermal amplification.
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SYTO-81 and SYTO-82 had the highest fluorescence enhancement.
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SYTO-81 had the widest range of working concentrations.
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These dyes were better suited for detection with simple optical setup (Gene-Z).
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Gene-Z was used to detect 10 copies of M. tuberculosis DNA in less than 10 min.