Reactive oxygen species production and antioxidant enzyme activity during epididymal sperm maturation in Corynorhinus mexicanus bats

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• 作者：Arenas-Rí ; os Edith^a ; ^{editharenas2000@yahoo.com.mx" class="auth_mail" title="E-mail the corresponding author} ; Rosado Garcí ; a Adolfo^a ; Corté ; s-Barberena Edith^b ; Kö ; nigsberg Mina^b ; Arteaga-Silva Marcela^a ; Rodrí ; guez-Tobó ; n Ahiezer^a ; Fuentes-Mascorro Gisela^c ; Leó ; n-Galvá ; n Miguel Angel^d
• 关键词：Antioxidant enzymes ; Bats ; Epididymis ; Sperm maturation ; ROS
• 刊名：Reproductive Biology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：16
• 期：1
• 页码：78-86
• 全文大小：746 K

文摘

Prolonged sperm storage in the epididymis of Corynorhinus mexicanus bats after testicular regression has been associated with epididymal sperm maturation in the caudal region, although the precise factors linked with this phenomenon are unknown. The aim of this work is to determine the role of reactive oxygen species (ROS) and changes in antioxidant enzymatic activity occurring in the spermatozoa and epididymal fluid over time, in sperm maturation and storage in the caput, corpus and cauda of the bat epididymis. Our data showed that an increment in ROS production coincided with an increase in superoxide dismutase (SOD) activity in epididymal fluid and with a decrease in glutathione peroxidase (GPX) activity in the spermatozoa in at different time points and epididymal regions. The increase in ROS production was not associated with oxidative damage measured by lipid peroxidation. The results of the current study suggest the existence of a shift in the redox balance, which might be associated with sperm maturation and storage.