Tissue expander stimulated lengthening of arteries (TESLA) induces early endothelial cell proliferation in a novel rodent model

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• 作者：Kristina Potanos^a ; ^{kristina.potanos@baystatehealth.org" class="auth_mail" title="E-mail the corresponding author} ; Nora Fullington^a ; ^{nora.fullington@umassmemorial.org" class="auth_mail" title="E-mail the corresponding author} ; Ryan Cauley^a ; ^{rcauley@partners.org" class="auth_mail" title="E-mail the corresponding author} ; Patricia Purcell^b ; ^{patty.purcellm@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; David Zurakowski^a ; ^{david.zurakowski@childrens.harvard.edu" class="auth_mail" title="E-mail the corresponding author} ; Steven Fishman^a ; ^{steven.fishman@childrens.harvard.edu" class="auth_mail" title="E-mail the corresponding author} ; Khashayar Vakili^a ; ^{khashayar.vakili@childrens.harvard.edu" class="auth_mail" title="E-mail the corresponding author} ; Heung Bae Kim^a ; ^{heung.kim@childrens.harvard.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Midaortic syndrome ; Middle aortic syndrome ; Artery lengthening ; Tissue expander ; Stretch ; Stress ; Tension
• 刊名：Journal of Pediatric Surgery
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：51
• 期：4
• 页码：617-621
• 全文大小：793 K

文摘

We examine the mechanism of aortic lengthening in a novel rodent model of tissue expander stimulated lengthening of arteries (TESLA).

Methods

A rat model of TESLA was examined with a single stretch stimulus applied at the time of tissue expander insertion with evaluation of the aorta at 2, 4 and 7 day time points. Measurements as well as histology and proliferation assays were performed and compared to sham controls.

Results

The aortic length was increased at all time points without histologic signs of tissue injury. Nuclear density remained unchanged despite the increase in length suggesting cellular hyperplasia. Cellular proliferation was confirmed in endothelial cell layer by Ki-67 stain.

Conclusions

Aortic lengthening may be achieved using TESLA. The increase in aortic length can be achieved without tissue injury and results at least partially from cellular hyperplasia. Further studies are required to define the mechanisms involved in the growth of arteries under increased longitudinal stress.